Journal: Cellular and Molecular Immunology

Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis

doi: 10.1038/s41423-025-01292-9

Figure Lengend Snippet: The synergistic interaction between LCN2 and TWEAK in keratinocytes. A Heatmap showing differential expressed genes after co-stimulating with TWEAK and LCN2 in keratinocytes. B Relative expression levels of IL6 , TNFa , IL17A, CCL5, CXCL5, CXCL10 and 24P3R were detected from HFKs using RT-qPCR. C , D Western blot was used to detect p-ERK1/2, p-JNK, p-p38, p-TRAF2, 24P3R and Fn14 protein level in keratinocytes after stimulation with LCN2, TWEAK, or both. One-way ANOVA was used for comparison between groups: ns ( P > 0.05); * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: CXCL10 (1:1000) were bought from GeneTex (GeneTex, Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Comparison

Journal: Cellular and Molecular Immunology

Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis

doi: 10.1038/s41423-025-01292-9

Figure Lengend Snippet: Lcn 2 –/– mice attenuate the pro-proliferative and inflammatory effects of IMQ on the epidermis. A After being treated with imiquimod and LCN2, the expression levels of Fn14, p-ERK1/2 and in the skin of wild-type mice were analyzed by Western blotting ( n = 3 per group). B Relative expression levels of Tnfsf12(Tweak) , Tnfrsf12a(Fn14) , Il6 , Tnfa , Il1b , Tgfb1 , Krt5 , Krt10 , and Krt17 from mice skin were detected using RT-qPCR. C , D Photographs of skin lesions in mice and the mouse psoriasis severity score difference between wild-type imiquimod mice and Lcn2 –/– imiquimod model mice. Body weight changes in mice modeled with imiquimod for six consecutive days. E Mouse serum levels of TWEAK were assayed by ELISA. F Western blotting was used to detect KRT1, KRT5, KRT6, KRT10, KRT14, KRT16, KRT17, Loricrin, Fn14, CXCL10, LCN2, p-ERK1/2, p-JNK, p-p38 in wild-type and Lcn2 –/– mice after treated with imiquimod ( n = 3 per group). G Relative mRNA expression levels of Lcn2 , Tnfsf12 , Tnfrsf12a , Il6 , Tnfa , Tgfb1 , Krt5 , Krt10 , Krt17 , 24p3r , Mc4r , Il1b were detected by RT-qPCR ( n = 3–5 per group). H Immunostaining of KRT5 and KRT14 were performed on paraffin sections. Data are shown as mean ± SEM ( n = 3–5 per group). ANOVA was used for comparison between groups: ns ( P > 0.05); * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: CXCL10 (1:1000) were bought from GeneTex (GeneTex, Shanghai, China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunostaining, Comparison